Insulin ELISA
Specification:
Catalog Number: Insulin HU-LB11
Description: Insulin ELISA
Sample Type: Serum
Sample Size: 10 µl
Available Sizes: 96 Wells
Range: 0-600 U/L
Sensitivity: 5.0 U/L
Incubation: 1 hour(s) 30 minutes (s)
Protocol: Insulin ELISA
Regulatory Status: RUO
Product Distribution: Available worldwide
The Life Biological Human Insulin ELISA employs a double monocolonal Ab sandwich ELISA format and is designed for the quantitative determination of insulin in human serum or plasma.
Insulin is a polypeptide hormone originating in the beta cells of the pancreas and serving as a principal regulator for the storage and production of carbohydrates. Its secretion is normally stimulated by increases in the amount of glucose in circulation. This leads to higher insulin levels and more rapid tissueassimilation of glucose followed by a decline in the insulin level as the glucose level subsides. In a number of conditions, notably insulinoma and diabetes, this relationship is impaired. Insulin tends to circulate at inappropriately high levels in patients with insulin-secreting pancreatic tumors, such tumors can thus be a cause of hypoglycemia. Accordingly, insulin immunoassays used sometimes in connection with provocative doses of tolbutamide or calcium play an essential role in the identification (and localization) of insulinomas. The finding of fasting hypoglycemia in association with an inappropriately high serum insulin concentration is considered diagnostic. Insulin levels do not figure in the subclassification of diabetes worked out by the National Diabetes Data Group. Nevertheless, when obtained in the course of a glucose tolerance test, they appear to be of some prognostic value in predicting the benefits of insulin therapy and the likelihood of progression to insulin-dependence and the complications (such as retinopathy) characteristic of diabetes.