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Prostate Specific Antigen

Catalog Number: PSA Total HU-LB22
Description: Prosthetic specific Antigen Total ELISA
Sample Type: Serum
Sample Size: 50 µl
Available Sizes: 96 Wells
Range: 0-100 ng/ml
Sensitivity: 0.5 ng/ml
Incubation: 2 hour(s) 20 minutes (s)
Protocol: Prosthetic specific Antigen Total ELISA
Regulatory Status: RUO
Product Distribution: Available worldwide

PSA (Total) ELISA - For the quantitative determination of the Cancer Antigen PSA concentration in human Serum. This kit is for research use only.
Prostate specific antigen (PSA), a member of the human kallikrein gene family, is a serine protease with chymotrypsin-like activity. The mature form of PSA is a single chain glycoprotein of 237 amino acids containing 7-8% carbohydrate as a single N-linked oligosaccharide side chain. PSA has a molecular weight of approximately 30,000 daltons.
The major site of PSA production is the glandular epithelium of the prostate. PSA has also been found in breast cancers, salivary gland neoplasms, periurethral and anal glands, cells of the male urethra, breast milk, blood and urine. PSA produced in the prostate is secreted into the seminal fluid in high concentrations. A major function of PSA is the proteolytic cleavage of gel-forming proteins in the seminal fluid, resulting in the liquification of the seminal gel and increased sperm mobility. Low levels of PSA are found in the blood as a result of leakage of PSA from the prostate gland. Increasing levels of serum PSA are associated with prostatic pathology, including prostatitis, benign prostatic hyperplasia (BPH), and cancer of the prostate.
PSA occurs in three major forms in blood. The major immunodetectable form is PSA complexed with the serine protease inhibitor, alpha-1-antichymotrypsin (PSA ACT). Uncomplexed, or free PSA, is the other immunodetectable form of PSA in serum. The majority of free PSA in serum appears to be an inactive form that cannot complex with protease inhibitors and may be either a PSA zymogen or an enzymatically-inactive, cleaved form of PSA.