FSH ELISA
Specification:
Catalog Number: FSH HU-LB28
Description: FSH ELISA
Sample Type: Serum
Sample Size: 50 µl
Available Sizes: 96 Wells
Range: 0-200 mIU/ml
Sensitivity: 2.0 mIU/ml
Incubation: 1 hour(s) 20 minutes (s)
Protocol: FSH ELISA
Regulatory Status: RUO
Product Distribution: Available worldwide

For the direct quantitative determination of Follicle Stimulating Hormone by enzyme immunoassay in human serum.
Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The (-subunit is similar to other pituitary hormones (luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (CG) ( while the (-subunit is unique. The (-subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary. In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis. In women, FSH acts on the granulosa cells of the ovary, stimulating steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in magnitude than LH) to its highest level. The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established . In this method, FSH calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal and enzyme labeled antibodies (directed against distinct and different epitopes of FSH) are added and the reactants mixed. Reaction between the various FSH antibodies and native FSH forms a sandwich complex that binds with the streptavidin coated to the well. After the completion of the required incubation period, the enzyme-Follicle Stimulating hormone antibody bound conjugate is separated from the unbound enzyme-follicle stimulating hormone conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce light. The employment of several serum references of known follicle stimulating hormone levels permits construction of a dose response curve of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with follicle stimulating hormone concentration.