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Testosterone ELISA

Testosterone (serum) ELISA
Catalog Number: Testosterone HU-LB16
Description: Testosterone  ELISA
Sample Type: Serum
Sample Size: 10 µl
Available Sizes: 96 Wells
Range: 0-16 ng/ml
Sensitivity: 0.09 ng/ml
Incubation: 1 hour(s) 15 minutes (s)
Protocol: Testosterone ELISA
Regulatory Status: RUO
Product Distribution: Available worldwide

Testosterone (Serum) ELISA is for the direct quantitative determination of Testosterone by enzyme immunoassay in human Serum.
Testosterone (17β-hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the β position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the
following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. The Testosterone ELISA kits are designed for the measurement of total Testosterone in human serum.